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OriGene
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Novus Biologicals
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Thermo Fisher
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Proteintech
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Protective Effect of Paeoniae Radix Alba Carbonisata on Hepatic Amyloidosis by Regulating Calcium Homeostasis
doi: 10.3390/ijms27062582
Figure Lengend Snippet: PRAC-E modulates calcium homeostasis via the cGMP/PKG/ATP2A1 signaling axis, thereby alleviating inflammation and amyloid deposition. ( A , B ) The dose-dependent ( A ) and time-dependent ( B ) effects of LYSO-6 on NCTC1469 cells. ( C ) Screening of the effective concentration of PRAC-E on LYSO-6-stimulated cells. ( D – F ) The levels of IL-6, TNF-α, and IL-8 in the cell culture supernatant. ( G – I ) The levels of SAA, SAP ( G ), and APO-E ( H ) in NCTC1469 cells. ( J ) CR staining of NCTC1469 cells. ( K ) The dose-dependent effects of BAPTA-AM on NCTC1469 cells. ( L , M ) Representative Fluo-4 AM fluorescence staining and semi-quantification of NCTC1469 cells. ( N ) Ca 2+ -ATPase activity in NCTC1469 cells. ( O ) Intracellular cGMP levels in NCTC1469 cells were measured by ELISA. ( P – R ) Western blotting and semi-quantitative analysis of PDE5A, PKG, and ATP2A1 in NCTC1469 cells. Data are presented as the mean ± SD ( n = 6). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. solvent control group; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. LYSO-6; ns, no significance.
Article Snippet: Rabbit antibodies against β-actin (Cat# 20536-1-AP), PDE5A (Cat# 22624-1-AP), and
Techniques: Concentration Assay, Cell Culture, Staining, Fluorescence, Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Solvent, Control
Journal: International Journal of Molecular Sciences
Article Title: Protective Effect of Paeoniae Radix Alba Carbonisata on Hepatic Amyloidosis by Regulating Calcium Homeostasis
doi: 10.3390/ijms27062582
Figure Lengend Snippet: Characterization of components in PRAC-E and analysis of core components. ( A , B ) Total ion flow diagram of PRAC-E under negative ( A ) and positive ( B ) ion mode. Number 1 to 42: The active ingredients are detailed in . ( C ) The types of compounds in the PRAC-E. ( D ) The TCM–component–target network. ( E ) Six core component structure diagrams. ( F ) Molecular docking binding energy. ( G ) Benzoyl paeoniflorin-ATP2A1. ( H ) Catechin-ATP2A1. ( I ) Gallate-ATP2A1. ( J ) Methylgallate-ATP2A1. ( K ) Paeoniflorin-ATP2A1. ( L ) Albiflorin-ATP2A1. Note: In G–L, the green font indicates non-ligand residue names, and the black font indicates hydrophobic residue names.
Article Snippet: Rabbit antibodies against β-actin (Cat# 20536-1-AP), PDE5A (Cat# 22624-1-AP), and
Techniques: Binding Assay, Residue